The accident occurred around on Friday evening when the 59-seater bus from neighbouring Henan province suddenly caught fire on a highway in Hanshou County in the city of Changde.
The bus had 53 passengers, two drivers and a tour guide at the time of the incident.
The two drivers were detained and an investigation into the cause of the accident is underway.
The incident came a day after 64 people lost their lives while 640 were injured in a powerful explosion at a chemical plant in eastern China. The blast occurred on Thursday following a fire in a fertilizer factory in a chemical industrial park in Yancheng, Jiangsu province, according to the government of Xiangshui county.